Josefa Alarcón-Apablaza; Pablo Navarro; Maria Cristina Manzanares Céspedes & Ramón Fuentes

SUMMARY: Tissue engineering aims to fabricate a scaffold that exhibits a suitable surface topography for a desired cellular response. Therefore, a study analyzing the characteristics of bone grafts is important for future research directions. This work aims to analyze the physical-chemical characteristics of commercially available bone grafts of human and bovine origin for dental use, using morphological analysis of the surface and chemical composition by variable pressure scanning electron microscope (VP- SEM) and energy-dispersive x-ray (EDX) spectrometry. In addition, pore diameter and surface area were analyzed by degassing method using a porosimeter, and particle size by laser diffraction. The analyzed allograft and xenograft particles differ in morphological characteristics and chemical composition. The allograft particles present a cuboidal and prismatic geometric morphology with angled edges and the absence of macropores. On the contrary, the xenograft particles present an irregular morphology with macropores in their structure. There is a statistically significant difference in C, P, and Ca between the xenograft and allografts (p < 0,05). The analyzed composition of allografts showed mainly the presence of C and O. In contrast, the composition of the xenograft was mainly Ca. These differences could influence the osteogenic properties of allografts and xenografts. This analysis provides basic information to understand the physicochemical properties of allografts and xenografts that facilitate cell-graft interaction.

KEYWORDS: Bone Regeneration, Bone Graft, Regenerative Medicine, Characterization, EDX, Morphology, Porosity, VP-SEM.

ALARCÓN-APABLAZA, J.; NAVARRO, P.; MANZANARES CÉSPEDES, M. C. & FUENTES, R. Analysis of the chemical composition, morphological characterization, and porosimetry of allograft and comparison with xenograft for dental applications. Int. J. Morphol., 42(3):698-708, 2024.