Refaat A. Eid; Mohamed Samir Ahmed Zaki; Mansour A. Alghamdi; Abulqasim Mohammed Sideeg; Kamal, Z. M. Ali; Mohamed Andarawi & Mohamed A. Haidara

This research was designed to investigate the potential protective effect of vitamin C supplementation against hepatocyte ultrastructural alterations induced by artemether (antimalarial drug) administration. Twenty-four adult male albino rats were used in this study and were divided into four groups (n=6). Group I served as a control and rats in group II administrated artemether (4 mg/kg B.W) orally for three consecutive days. Group III administered artemether plus a low dose of vitamin C (2.86 mg/kg/l water) while group IV received artemether plusa high dose of vitamin C (8.56 mg/kg). At the end of the experimental period (14 days), the harvested liver tissues were examined by transmission electron microscopy (TEM), and blood samples were assayed for biomarkers of liver injury and oxidative stress. Artemether significantly (p<0.05) augmented biomarkers of liver injury such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and oxidative stress such as superoxide dismutase (SOD), Glutathione Peroxidase (GPX), and caused degeneration and damage of the rough endoplasmic reticulum and disrupted mitochondria. The blood sinusoids were also damaged with distortion of their canaliculi. Administration of vitamin C showed improvement of liver biomarkers, and liver parenchyma, especially in a high dose of vitamin C.We concludes that vitamin C is a partial protective agent against artemether-induced liver injury.

KEY WORDS: Artemether; Rats; Vitamin C; Hepatocyte ultrastructure; Biomarkers liver injury; Oxidative stress.

EID, R. A.; ZAKI, M. S. A.; ALGHAMDI, M. A.; SIDEEG, A. M.; ALI, K. Z. M.; ANDARAWI, M. & HAIDARA, M. A. Vitamin C administration attenuated Artemether-induced hepatic injury in rats. Int. J. Morphol., 38(1):48-55, 2020.