Osvaldo Macías Marín; Alma Lilian Guerrero Barrera; Arturo Gerardo Valdivia Flores; Teodulo Quezada Tristan; Francisco Javier Avelar Gonzalez; María Magdalena Soto Perezchica & Alfredo Salazar de Santiago

The domestic chicken is a species of bird that has been extensively studied in regard to its biology and as a model organism for science. The reproduction of the species is by the laying of fertilized eggs, which in a period of 21 days will develop a chick inside. Several methods have been described to develop embryos ex-ovo, allowing the observation and manipulation of the organism. This work has the propose to standardize a method that allows the development of the embryos inside the artificial incubation system, which has a low cost and is easy to make. In this work, 100 chicken eggs were used to study the effects of humidity, mineral supplementation, and the preincubation time of the egg on the incubation ex-ovo of the embryos. Embryo development was documented through the different days. Pulverized eggshell was selected as an optimal source to provide calcium, magnesium, phosphorus, and other minerals to the developing embryo. By providing 900-1200 mg of pulverized eggshell, 40 mL of the 0.001 % solution of benzalkonium chloride, and a preincubation time of approximately 56 h, the embryos were able to develop until 19 days, and even though they did not reach hatching, the incubation conditions that allowed the survival and development of embryos until late stages were achieved. Thus, due to the conditions established for calcium, humidity and preincubation time, in the present work, the chicks reached 19 days of development.

KEY WORDS: Birds; Embryonic development; ex-ovo incubation; Gallus gallus domesticus; incubation conditions.

MACÍAS, M. O.; GUERRERO, B. A. L.; VALDIVIA, F. A. G.; QUEZADA, T. T.; AVELAR, G. F. J.; SOTO, P. M. M. & DE SANTIAGO, A. S. Study of ex-ovo embryonic development of Gallus gallus domesticus. Int. J. Morphol., 41(2):668-674, 2023.