Premrudee Hemha; Surang Chomphoo; Yada Polsan; Kaoru Goto; Hisatake Kondo & Wiphawi Hipkaeo

Diacylglycerol kinase (DGK) exerts balancing the intracellular level between two-second messengers, diacylglycerol and phosphatidic acid, by its phosphorylation activity. DGK ζ is often localized in cell nuclei, suggesting its involvement in the regulation of intranuclear activities, including mitosis and apoptosis. The present immunohistochemical study of rat kidneys first revealed no detection levels of DGK ζ -immunoreactivity in nuclei of most proximal tubule epithelia in contrast to its distinct occurrence in cell nuclei of collecting and distal tubules with the former more dominant. This finding suggests that DGK ζ is a key factor regulating vulnerability to acute kidney injury in various renal tubules: its low expression represents the high vulnerability of proximal tubule cells, and its distinct expression does the resistance of collecting and distal tubule cells. In addition, this isozyme was more or less localized in nuclei of cells forming glomeruli as well as in endothelial nuclei of peritubular capillaries and other intrarenal blood vessels, and epithelial nuclei of glomerular capsules (Bowman’s capsules) and renal calyces, including intrarenal interstitial cells.

KEY WORDS: DGK ζ ; Kidney; Rat; Nucleus; Renal tubule.

HEMHA, P.; CHOMPHOO, S.; POLSAN, Y.; GOTO, K.; KONDO, H. & HIPKAEO, W. Localization of diacylglycerol kinase ζ in the kidney of adult rats: its dominant expression in collecting tubules and its selective lack in proximal tubules. Int. J. Morphol., 41(3):789- 797, 2023.