Ismael Vilella; Eva M García.; Jorge Ten; Rafael Bernabeu; Yolanda Segovia Huerta; María José Gómez-Torres

Summary

To know the molecular aspects that occur in the process of human sperm binding to the human zona pellucida (ZP) is one of the great challenges of reproduction biology. Moreover knowing if the fertilization process may be affected by cryopreservation of female gametes is still another issue discussed in the literature. Based on this, the main objective of this study was to determine whether the oocyte vitrification may alter the interaction of sperm with the glycocalyx of ZP and show whether these oocytes lost the ability to induce the acrosome reaction in sperm. According to our results the oocyte closed vitrification method (S3) does not alter the ability of the sperm binding to the zona pellucida, and their ability to induce the acrosome reaction.

KEY WORDS: Hemizona test; Human oocytes; Acrosome reaction; Vitrification; Human sperm.

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