The morphological aspects of tendon and muscle component of the posterior compartment have been described in some population groups. The morphological and biometric characteristics of the posterior compartment of 50 thighs of cadaverous specimens were evaluated by means of a direct design, in a sample of the Colombian population. The 2 % of the hamstring beats originated in a common tendon, 58 % with a common tendon for the Semitendinous muscle (MSt) and long head of the femoral Biceps muscle (MBF). The 40 % of the long head of the MBF were originated on the lateral margin of the Ischial Tuberosity (TI) and the MSt on its posterior surface. In 98 % of the cases the semimembranous muscle (MSm) originated in the middle margin of the IT and dissolved as a medial structure of the thigh. No variation was observed in the place of the hamstring insertion. The longest time (419.5 DE 33.2 mm) the distal tendon with greater thickness was that of the MBF (8.5 OF 5.6 mm), while the semimembranosus muscle (MSm) presented the broadest myotendinous component (22.4 SD 6.5 mm). There weren’t statistically significant morphometric differences in relation to the presentation side. The findings obtained enrich the morphological concepts of the posterior thigh region, contribute to the qualification of plastic surgery, orthopedic procedures and the design of muscle training programs in different sports disciplines.