Sandra Trivunic Dajko; Jovo Bogdanovic; Bojana Andrejic Visnjic; Milan Popovic; Matilda Djolai & Ondrej Hes

The group of primary renal tumours with granular-oncocytic cytoplasm is a very heterogeneous group, in its histological origin and biological behavior resulting in many diagnostic problems. In this study 57 renal epithelial tumours with granular oncocytic cells were analyzed using fluorescence in situ hybridisation (FISH), array comparative genomic hybridisation (aCGH) and polymerase chain reaction (PCR). The results of analysis in renal oncocytoma (RO) did not indicate the presence of the gene mutations or chromosomal abnormalities. Sporadic renal hybrid oncocytic/chromophobe tumours (HOCT) had multiple numerical aberrations of chromosomes 1, 2, 6, 9, 10, 13, 17, 20, 21 and 22. This type of tumour had no mutations in the VHL, c-kit, PDGFRA, and FLCN genes. Oncocytic papillary renal cell carcinoma (O-PRCC) had numerical abnormalities of chromosomes 7 and 17 and the loss of the Y chromosome. Cytogenetic analysis of 20 pigmented microcystic chromophobe renal cell carcinomas (PMChRCC) showed monosomy as the most frequent aberration in all analyzed chromosomes 1, 2, 5, 10, 13, 17 and 21. One case of chromophobe renal cell carcinoma (ChRCC) with hyaline globules had a mutation in the distal part of exon 3 of the VHL gene. Absence of genetic disorders in usual RO is common result, but we have established absence of genetic disorders even in rare variants. Variety of genetic alterations detected in sporadic renal HOCT proves it to be a separate entity, not a variant of ChRCC, while PMChRCC is an uncommon variant of ChRCC. O- PRCC is a subtype of papillary renal cell carcinoma.

KEY WORDS: Renal tumours; Granular oncocytic cytoplasm; Molecular genetic analysis.

TRIVUNIC DAJKO, S.; BOGDANOVIC, J.; ANDREJIC VISNJIC, B. A.; POPOVIC, M. ; DOJLAI, M. & HES, O. Molecular genetic analysis of primary renal epithelial tumours with granular oncocytic cytoplasm. Int. J. Morphol., 39(6):1719-1726, 2021.