Xing Zhou; Zhixue Wang; Min Tang; Duoyun Gong; Ya Luo; Fei Liu & Liqun Zhang

This study is to investigate the immunomodulatory and anti-cancer effects of the herbal formula of Polygonatum sibiricum polysaccharide (PSP), pachymaran (P) and Astragalus polysaccharide (APS) in mouse models of immunosuppression and lung cancer. The immunosuppressive model was established in Kunming mice, which were divided into normal control group, immunosuppressive model group and PSP+P+APS group. The lung cancer model was established inC57 mice with Lewis cells. These mice were divided into normal control, lung cancer model, PSP+P+APS, and ADM group. Peripheral white blood cells (WBC) were detected with an automatic blood cell analyzer. Percentage of peripheral blood CD4+ and CD8+Tlymphocytes were detected by flow cytometry. Spleen and thymus indices and tumor inhibition rate were calculated. Serum levels of TNF, IFN-γ, IL-4, IL-6, IL-10, andIL- 17A were detected with the BD cytometric bead array. Compared with the respective model group, administration of the herbal formula PSP+P+APS group significantly increased the number of WBC, thymus index, spleen index, CD4+/CD8+ratio, TNF, IFN-γ, and IL- 17Afor both the immune suppressive model and lung cancer model mice (P<0.05). On the contrary, the levels of IL-6 and IL-10 were significantly decreased in the PSP+P+APS group (P<0. 05). In addition, the herbal formula significantly reduced the tumor weight of the lung cancer model mice. The PSP+P+APS herbal formula plays an important role in the immunomodulation and tumor inhibition in the mice with immunosuppression and lung cancer.

KEY WORDS: Polygonatum sibiricum polysaccharide; Pachymaran; Astragalus polysaccharide; Herbal formula; Lung cancer.

ZHOU, X; WANG, Z.; TANG, M.; GONG, D.; LUO, Y.; LIU, F. & ZHANG, L. Herbal formula of Polygonatum sibiricum polysaccharide, pachymaran and astragalus polysaccharide exert immunomodulatory effects in mouse models of immunosuppression and lung cancer. Int. J. Morphol., 43(1):326-334, 2025.