Ming Xu & Xingyu Zhu

Summary

Several wound-dressing materials have functional and structural flaws that limit them from facilitating wound healing. Accelerating blood coagulation, preventing bacterial infection, and starting the regeneration process in full-thickness wounds are all possible outcomes of the design of multifunctional wound dressings. Herein, Hippophae rhamnoides L (HRL), a traditional Chinese herb, was combined with substituted hydroxyapatite (SHA)/alginate (ALG)/carboxymethyl cellulose (CMC) (HSAC) via freeze- drying, electrostatic attraction, and ecofriendly crosslinking techniques to make multifunctional bioscaffold sponges. HSAC samples cell proliferation was highly enhanced compared to control for different cell culture times. While in vivo study was carried out using an excisional wound model, in vitro investigations were carried out utilizing toxicity and wound healing assays. Histology was performed on tissues extracted from the wound region. We observed the wound healing process throughout the one, three, and fourteen days. The HRL-containing bioscaffold sponge formulation performed better than the control group when wound construction was evaluated. Histological analysis showed that the HRL formulation induced skin regenerative activity. The wound healing experiment utilizing HUVEC cells revealed significant cell proliferation when HRL-bioscaffold sponges were used. Finally, both ex vivo and in vivo, our test material HSAC bioscaffold sponges significantly accelerated wound healing. KEY WORDS: Bioscaffold; Antibacterial activity; Burn wound; Herbal; Wound healing.

How to cite this article

XU, M. & ZHU, X. Bioscaffold sponge with Hippophae rhamnoides L accelerates burn wound repair: In vitro and in vivo studies. Int. J. Morphol., 43(2):351-359, 2025.