Nawel Morzouglal; Hanane Bouchefa; Amel Ghozlani & Fatima Hadj-Bekkouche

The evolution of food production causes physiological diseases in the body, an important industrial product is fructose; affecting the organ most sensitive to these products, the pancreas. At the pancreatic level, thyrotropic hormone (TRH) has membrane receptors in the β-cell; subsequently, TRH stimulates and regulates insulin release and participates in the regulation of hyperglycemia. The aim of this study is to demonstrate the effect of TRH in cases of pancreatic metabolic disorders and to provide a new vision for the prevention and/or treatment of diabetes. The investigation was carried out using Wistar rats. Animals were devised on four groups: control, TRH, Fru and Fru -TRH. At the age of 25-day, animals (Fru, Fru -TRH) received Fructose (20 %) and control groups received water; at the age of 60 days (TRH and Fru -TRH groups), the treatment with intraperitoneal injections of TRH for 7 days, control rats received the same volume of saline solution. Pancreases were collected, histologically studied and stained with eosin/ haematoxylin (structural study) and fuchsin/paraldehyde (histochemical study); biochemical parameters, insulin levels, catalase activities, malondialdehyde (MDA) and inflammatory marker NO were measured. We found that TRH corrected metabolic parameters and inflammatory markers in the islets by suppressing cellular infiltration and cytoplasmic vacuolation. These results suggest that the direct action of TRH on β-cells, due to the presence of its receptor at the membrane level, can modulate tissue and functional alterations of these cells in pathological cases.

KEY WORDS: TRH; Fructose; b-cells; Oxidative stress; Rat.

MORZOUGLAL, N.; BOUCHEFA, H.; GHOZLANI, A. & HADJ-BEKKOUCHE, F. Thyrotropin-releasing hormone and corrective effect of fructose-induced metabolic disorders on pancreatic b-cell function in wistar rats. Int. J. Morphol., 43(3):986-992, 2025.