Yuwei Gao; Xinyue Wang; Xianghui Li; Tingting Shi; Zhaoting Kou & Chun Yang
Inflammatory response plays an important role in tissue repair and regeneration. The tail of Scincella tsinlingensis was amputated with a razor blade to set up tail regeneration model. Specific cell markers of myeloid-derived monocytes, mast cell, T cell and B cell determined by single-cell transcriptome analysis of Anolis carolinensis were used to detect the spatial and temporal distribution of CD34+ monocytes, CMA1+ mast cells, CCR7+ T lymphocytes and CD22+ B lymphocytes in the immunoinflammatory stage of tail regeneration in S. tsinlingensis to bring further support to the idea of immune regulation of tail regeneration in reptiles. The results revealed distinct spatial localization patterns among different immune cell populations. Notably, CCR7+ T cells were absent from both the wound site and the dermis of proximal scales adjacent to the amputation surface, whereas CMA1+ mast cells were ubiquitously distributed across these regions. CD34+ monocytes and CD22+ B cells exhibited selective localization, being exclusively observed in the dermal layer of proximal scales near the amputation interface. Temporal analysis (0.5-7 days post-amputation, dpa) demonstrated dynamic recruitment patterns. All four immune cell types infiltrated both the wound bed and proximal dermal regions during early regeneration stages. Immune cell infiltration peaked at 5 dpa, followed by significant reduction by 7 dpa. These temporal dynamics suggest that myeloid-derived immune cells are initially recruited to the injury site through inflammatory signaling cascades, then diminish as inflammation resolves, potentially facilitating the transition from inflammatory response to regenerative processes in S. tsinlingensis.
KEY WORDS: Scincella tsinlingensis; Tail regeneration; Wound healing; Myeloid-derived immune cells.
GAO, Y.; WANG, X.; LI, X.; SHI, T.; KOU, Z. & YANG, C. The spatial-temporal distribution of myeloid-derived immune cells in the wound healing stage of regenerating tail in Scincella tsinlingensis. Int. J. Morphol., 43(5):1624-1634, 2025.
