Al-Amri, I. S.; Al-Mawali, M. J.; Doost Mohammadi, H. S.; Farhadi, S.; AlKindi, A. Y.; Al-Hashmi, S. A.; Al-Harrasi, A. S.; Abbas, G.; Kadim, I. T.; Khalaf, S. K.; Ur Rehman, N.; Shehla, A.; Jamshidi-Adegani, F.; Vakilian, S.; Al-Amri, A. I. & Shaikh, J.
Diabetes mellitus (DM) causes immune dysfunction and spleen damage. This study investigated the therapeutic effects of 11-keto-β-boswellic acid (KBA) isolated and semi-synthesized from Boswellia sacra resin and commercially sourced 5- chloro-8-hydroxyquinoline (CHQ) on spleen injury in streptozotocin-induced diabetic mice. Twenty-four female CD1 mice were divided into control, diabetic, diabetic + CHQ, and diabetic + KBA groups. KBA (25 mg/kg/day) or CHQ (25 mg/kg/day) was administered intraperitoneally for four weeks. Spleen tissues were examined histologically with hematoxylin–eosin staining and morphometric analysis. Diabetic mice showed noticeable capsular thickening, disruption of white pulp architecture, lymphocyte depletion, and red pulp congestion. Both treatments significantly improved splenic structure; however, KBA treatment produced near-normal restoration of white and red pulp morphology, reduced inflammation, and lowered siderophage accumulation compared with CHQ. Laboratory-isolated KBA demonstrated superior protection against diabetes-induced splenic dysfunction, highlighting its potential as a novel therapeutic approach for immune complications associated with diabetes. Further studies are warranted to define the underlying molecular mechanisms.
KEY WORDS: 11-Keto-β-boswellic acid; 5-Chloro-8-hydroxyquinoline; Spleen; Diabetes mellitus; Histopathology.
AL-AMRI, I. S.; AL-MAWALI, M. J.; DOOST MOHAMMADI, H. S.; FARHADI, S.; ALKINDI, A. Y.; AL-HASHMI, S. A.; AL- HARRASI, A. S.; ABBAS, G.; KADIM, I. T.; KHALAF, S. K.; UR REHMAN, N.; SHEHLA, A.; JAMSHIDI-ADEGANI, F.; VAKILIAN, S.; AL-AMRI, A .I. & SHAIKH, J. Therapeutic efficacy of 11-keto-β-boswellic acid and 5-chloro-8-hydroxyquinoline in ameliorating spleen dysfunction in streptozotocin-induced diabetic mice. Int. J. Morphol., 44(2):471-479, 2026.
